解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The organization of mouse immunoglobulin heavy chain genes has been investigated by hybridization with cloned mu and alpha cDNA probes. Restriction endonuclease fragments bearing mu and alpha constant region genes and two types of variable region (VH) genes were compared in BALB/c embryos, liver and nine plasmacytomas...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(80)90386-4
更新日期:1980-01-01 00:00:00
abstract::The mRNAs from two yeast mitochondrial genes cob-box (cytochrome b) and oxi-3 (cytochrome oxidase 40,000 dalton subunit) are processed from large (7-10 kb) precursors. Certain mutations in each gene block the maturation of the RNAs from both genes at a variety of specific steps. The pleiotropic cytochrome b mutants se...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90233-2
更新日期:1979-12-01 00:00:00
abstract::Nuclear matrices from undifferentiated and differentiated Friend erythroleukemia cells have been obtained by a method which removes DNA in a physiological buffer. These matrices preserved the characteristic topographical distribution of condensed and diffuse "chromatin" regions, as do nuclei in situ or isolated nuclei...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90221-6
更新日期:1979-12-01 00:00:00
abstract::A cDNA library has been constructed from an RNA preparation highly enriched in silkmoth chorion mRNAs. Many distinct clones have been identified from this library using a stepwise procedure: scoring for infrequent hexanucleotide restriction enzyme recognition sequences; detailed characterization with restriction enzym...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90241-1
更新日期:1979-12-01 00:00:00
abstract::ATP increases microtubule steady state assembly and disassembly rates in vitro in a concentration-dependent manner. Bovine brain microtubules, composed of 75% tubulin and 25% high molecular weight microtubule-associated proteins (MAPs), were purified by three cycles of assembly and disassembly in the absence of ATP. W...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90122-3
更新日期:1979-11-01 00:00:00
abstract::Following EMS mutagenesis we recovered a mutant of D. melanogaster that grows at concentrations of alpha-amanitin lethal to wild-type. To our knowledge this mutant represents the first example of an amanitin-resistant eucaryotic organism. The amanitin resistance of the mutant (AmaC4) is due to an alteration in its DNA...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90116-8
更新日期:1979-11-01 00:00:00
abstract::The mammalian alpha- and beta--globin genes are thought to have evolved from a common ancestral sequence by a duplication event that occurred over 500 million years ago. We have now determined the entire nucleotide sequence of a cloned mouse alpha--globin gene, including regions that flank and interrupt the coding seq...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90139-9
更新日期:1979-11-01 00:00:00
abstract::Radioactive alpha factor is degraded to discrete biologically inactive fragments by the target a cells of S. cerevisiae, but not by alpha cells which make the pheromone. The pattern of cleavage products and sequence analysis of one fragment indicated that the first scission occurred between leucine 6 and lysine 7. The...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90117-x
更新日期:1979-11-01 00:00:00
abstract::During the early stage of Ad2 infection of human cells, RNA is transcribed from five separate transcription units. Early region II encodes the mRNA for a 72K single-stranded DNA binding protein (DBP) which functions in DNA replication. This report describes the structure of the first leader of the DBP mRNA and the fla...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90073-4
更新日期:1979-10-01 00:00:00
abstract::We describe genetic and physical characterization of rearrangements of chromosome III which result in changes of cell type in S. cerevisiae. Two types of rearrangements were obtained as rare events which caused a change at the locus controlling cell type, MAT, associated with a recessive lethal mutation, in one case f...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90050-3
更新日期:1979-10-01 00:00:00
abstract::We have studied the interaction of highly purified Int protein with DNA restriction fragments from the lambda phage attachment site (attP) region. Two different DNA sequences are protected by bound Int protein against partial digestion by either pancreatic DNAase or neocarzinostatin. One Int binding site includes the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90049-7
更新日期:1979-10-01 00:00:00
abstract::In this study, we have located the sites of transcription initiation and termination on a cloned fragment of ribosomal DNA from X. laevis, and have sequenced the surrounding nucleotides. As reported previously (Reeder, Sollner-Webb and Wahn, 1977), about 25% of the 40S rRNA precursor molecules isolated from oocytes ha...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90066-7
更新日期:1979-10-01 00:00:00
abstract::We have isolated a conditional lethal mutant of bacteriophage 12 which makes plaques only on E. coli strains carrying a UGA suppressor. It grows normally in nonsuppressing hosts but does not lyse such strains. The mutation complements with amber mutations in each of the three known phage cistrons. These observations l...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90043-6
更新日期:1979-10-01 00:00:00
abstract::We studied H1 gene expression in hybrids of Xenopus laevis (female) x Xenopus borealis (male) and saw paternal H1 synthesis in mid-blastulae, which indicates that the H1 genes are active by this stage. The behavior of the maternal store of H1 histone mRNA was studied in androgenetic haploids. In which all stored mRNA ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90365-9
更新日期:1979-09-01 00:00:00
abstract::Certain tRNAs in S. cerevisiae (tRNATyr and tRNAPhe) arise via precursor molecules which are mature at the 5' and 3' termini but contain intervening sequences adjacent to the anticodon (Knapp et al., 1978; O'Farrell et al., 1978). In addition to these molecules, precursors to several other tRNAs accumulate in a temper...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90349-0
更新日期:1979-09-01 00:00:00
abstract::Pure primary mesenchymal cells from definitive streak stage chick embryos have been prepared free of epiblast and hypoblast cells. These cells have the potential in culture to differentiate into erythroid cells, beating heart muscle tissue, chondrocytes and epithelial cells. Transformation in vitro of pure primary mes...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90320-9
更新日期:1979-08-01 00:00:00
abstract::Specific active transcription units on chromosome IV in the salivary glands of Chironomus tentans have been visualized by the Miller spreading technique and in situ by conventional electron microscopy. These units are likely to be located in the two most conspicuous puffs on chromosome IV, Balbiani ring 1 (BR 1) and B...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90324-6
更新日期:1979-08-01 00:00:00
abstract::When human lymphoblastoid mRNA was microinjected into X. laevis oocytes, titers of interferon rapidly reached a maximum inside the oocyte while accumulation of interferon continued in the incubation medium for at least 45 hr. If interferon protein was injected into oocytes it was rapidly inactivated. Significantly, ne...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90260-5
更新日期:1979-07-01 00:00:00
abstract::The nucleotide sequences of the 3' termini of the DNA from four autonomous rodent parvoviruses have been determined. The terminus of each genome exists as a Y-shaped hairpin structure involving 115 or 116 nucleotides. The sequence of this region of DNA is highly conserved and shows no evidence of internal sequence het...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90276-9
更新日期:1979-07-01 00:00:00
abstract::The sequence of about one third of the polyoma virus genome is presented. This sequence covers the origin of replication of two large plaque strains (A2 and A3) of polyoma virus. The two strains differ by 11 bp in the origin region. A model for replication is suggested. The sequence probably also covers the entire cod...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90162-4
更新日期:1979-06-01 00:00:00
abstract::The messenger RNA coding for the precursor of the small subunit of ribulose--1,5--bisphosphate (Ru-P2) carboxylase has been partially purified from pea leaves. The mRNA has a size of 11.5S, is approximately 800 nucleotides long and, on cell-free translation, directs the synthesis of a single major polypeptide of 20,00...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90164-8
更新日期:1979-06-01 00:00:00
abstract::A gene for Saccharomyces cerevisiae tRNATrp has been sequenced which contains an intervening sequence of 34 bp (H. S. Kang and J. Abelson, unpublished results). The mutant yeast strain ts-136 accumulates a precursor to tRNATrp which contains mature ends and is colinear with the tRNATrp gene. A nuclear extract from Xen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90166-1
更新日期:1979-06-01 00:00:00
abstract::To determine the special feature of ribosomal RNA promoters that might account for the highly efficient and regulated synthesis of rRNA in E. coli, we have analyzed the beginnings of two ribosomal RNA operons, rrnD and rrnX. DNA sequences for 425 bp preceding those specifying mature 16s rRNA are reported. In vitro tra...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90310-6
更新日期:1979-05-01 00:00:00
abstract::Study of Asians has previously indicated that deletion of alpha-globin structural genes is the predominant lesion in alpha-thalassemias and that Hb H disease occurs when three of four normal alpha loci per cell are deleted. To test the generality of this model, Hb H disease DNAs of both Asian and non-Asian origin were...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90292-7
更新日期:1979-05-01 00:00:00
abstract::After three or more passages, stromal cell outgrowths from explants of normal rabbit corneas and from corneas previously burned with alkali did not produce collagenase until they were stimulated by the addition of rabbit blood-derived mononuclear cells or media conditioned by them. Normal stromal cells required stimul...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90104-1
更新日期:1979-04-01 00:00:00
abstract::We have compared the chromatin structure in the active and inactive states at loci encoding the major heat shock protein in Drosophila. DNAase I and micrococcal nuclease were used as probes of higher order organization and nucleosomal integrity. Such integrity is gauged here by the characteristic pattern of discrete D...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90096-5
更新日期:1979-04-01 00:00:00
abstract::We have identified two major and approximately ten minor poly(A)-containing RNA species in S. cerevisiae which arise from in vivo transcription of the yeast plasmid, known as 2mu circle. The two major species, which are 1325 and 1275 bases in length, are transcribed from the two unique halves of the plasmid and extend...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90098-9
更新日期:1979-04-01 00:00:00
abstract::This paper describes a model for the structure of DNA contained in three morphologically similar bacteriophages--T7, P22 and phiCd-1--based on the transient electric dichroism of intact phage. The reduced dichroism of each of the phages at perfect orientation is within the range +0.12 to +0.19. Assuming that the phage...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90026-6
更新日期:1979-03-01 00:00:00
abstract::The majority of cell lines derived by infection of murine bone marrow cells with Abelson murine leukemia virus (A-MuLV) synthesize a mu chain but no detectable light chain. Aside from this mu-only phenotype, lines that make only light chain, both chains or no immunoglobulin-related polypeptides have also been found. T...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90014-x
更新日期:1979-02-01 00:00:00
abstract::We have determined the nucleotide sequence of a secondary lambda attachment site in trpC. Direct sequence analysis of lambdatrp transducing phage DNA fragments carrying the two prophage attachment sites reveals a 6 nucleotide homology in the crossover region which is a subset of the 15 nucleotide core sequence in the ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90016-3
更新日期:1979-02-01 00:00:00
abstract::Two allelic forms of the natural chicken ovalbumin gene have been independently cloned. These alleles differ from each other by an Eco RI restriction cleavage site in one of the seven intervening sequences within the natural ovalbumin gene. Restriction endonuclease mapping and sequence analyses of these cloned genotyp...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(79)90201-0
更新日期:1979-01-01 00:00:00
abstract::Extraction of glycerinated chicken skeletal muscle with 0.6 M potassium iodide leaves a framework of insoluble components within each muscle fiber. This framework is composed primarily of planes of in-register Z discs that have been thickened by the accumulation of material on both sides of each disc during extraction...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90051-x
更新日期:1978-12-01 00:00:00
abstract::Genomic Xenopus borealis oocyte-specific 5S DNA (Xbo) contains clusters of 5S rRNA genes. The number of genes varies among clusters, and the distance between genes within a cluster is about 80 nucleotides. The spacer DNA between gene clusters is AT-rich and heterogeneous in length due in part to variable numbers of a ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90042-9
更新日期:1978-12-01 00:00:00
abstract::Temperature-sensitive mutants of vesicular stomatitis virus (VSV) belonging to complementation group III contain a lesion in the matrix (M) protein. This results in a 2--5 fold increase in transcription at the nonpermissive temperature. Co-infection of cells with one of these mutants and wild-type virus reverses this ...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90069-7
更新日期:1978-12-01 00:00:00
abstract::We have determined the nucleotide sequences of the germ line gene as well as a corresponding somatically mutated and rearranged gene coding for a mouse immunoglobulin lambdaI type light chain. These sequencing studies were carried out on three Eco RI-DNA fragments which had been cloned from BALB/c mouse embryos or a l...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90041-7
更新日期:1978-12-01 00:00:00
abstract::Cholera toxin inhibition of progesterone-induced meiosis of Xenopus laevis oocytes in vitro has been correlated with increased cAMP levels. Inhibition of germinal vesicle breakdown (Gvbd) and cAMP increase occurred after a lag period of 2 hr, when cholera toxin was injected, or 4--5 hr, when applied externally. The ab...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90052-1
更新日期:1978-12-01 00:00:00
abstract::Linear simian virus 40 (SV40) chromosomes were prepared by Eco R1 nuclease cleavage of the circular SV40 chromosomes released from virions with dithiothreitol at pH 9,8. Chromatin-DNA hybrids were constructed with segments of 3H-labeled, naked SV40 DNA covalently joined via the Eco R1-generated cohesive ends to segmen...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90279-9
更新日期:1978-11-01 00:00:00
abstract::A temperature-sensitive mutant of BHK, designated ts BN-2, shows a rapid drop in 3H-thymidine incorporation along with accumulation of the cells in the G1 phase of the cycle when asynchronous cultures are shifted from 33.5 degrees C to the nonpermissive temperature of 39.5 degrees C. Synchronized cultures of ts BN-2 c...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90017-x
更新日期:1978-10-01 00:00:00
abstract::Transfection experiments with HSV 1 in which one uses herpes simplex virus (HSV) thymidine kinase (TK) as a selectable prototrophic marker yield two classes of transformed cells: stable and unstable. In this report, we test the hypothesis that the stability phenotype can be explained by virus genome integration into a...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90015-6
更新日期:1978-10-01 00:00:00
abstract::Cells producing avian sarcoma virus (ASV) contain at least three virus-specific mRNAs, two of which are encoded within the 3' half of the viral genome. Each of these viral RNAs can hybridize with single-stranded DNA(cDNA5') that is complementary to a sequence of 101 nucleotides found at the 5' terminus of the ASV geno...
journal_title:Cell
pub_type: 杂志文章
doi:10.1016/0092-8674(78)90084-3
更新日期:1978-09-01 00:00:00